Cloning, expression and pharmacological characterisation of the mouse somatostatin sst5 receptor

Abstract

The mouse somatostatin (somatotropin release inhibiting factor, SRIF) sst5 receptor coding sequence was cloned from a mouse BALB/c genomic library. It shows 97% and 81% homology with the corresponding rat and human receptors, respectively. The msst5 receptor messenger RNA (mRNA) is present at low levels in the adult mouse brain, with significant expression in a few nuclei only, e.g. in the septum (lateral septal nuclei) or the amygdala (medial amygdaloid nucleus); very few signals were observed in the mesencephalon, metencephalon, and myelencephalon (except the dorsal motor nucleus of the vagus nerve).The msst5 receptor was stably expressed in the hamster fibroblast cell line CCL39-SRE-Luci,..