Journal article

NONSPECIFIC UPTAKE OF THE RADIOLIGAND I-125-LABELED IHYP BY INTACT HUMAN-LYMPHOCYTES - REVERSAL OF THE UPTAKE PROCESS

E PAIETTA, D HOYER, G ENGEL, JD SCHWARZMEIER

MOLECULAR AND CELLULAR ENDOCRINOLOGY | ELSEVIER SCI IRELAND LTD | Published : 1982

Abstract

Binding of (+/-)[125I]iodohydroxybenzylpindolol (IHYP) to beta-adrenoceptors on intact human peripheral blood lymphocytes was found to be associated with a high degree of non-specific intracellular uptake of the radioligand. Non-specific binding on cellular surfaces was identified by displacing IHYP from its specific binding sites with the cold competing antagonist (+/-)-propranolol. Another methodological approach, however, was necessary to eliminate the uptake problem: release of intracellularly accumulated IHYP was achieved by sedimentation of the IHYP-loaded cells and resuspension in hypotonic buffer (HME buffer) for 10 min at +4 degrees C. The true value of maximal IHYP binding measured..

View full abstract

University of Melbourne Researchers