Journal article

SEROTONIN INCREASES THE PRODUCTION OF INOSITOL PHOSPHATES AND MOBILIZES CALCIUM VIA THE 5-HT2 RECEPTOR IN A7R5 SMOOTH-MUSCLE CELLS

VM DOYLE, JA CREBA, UT RUEGG, D HOYER

NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY | SPRINGER VERLAG | Published : 1986

Abstract

Serotonin (5-HT) induces inositol phosphate production and the efflux of 45Ca2+ in a smooth muscle cell line (A7r5) derived from rat aorta. These effects were pharmacologically characterised and compared to data obtained in radioligand binding studies performed with the 5-HT2 ligand [3H]ketanserin in rat brain cortex membranes. 5-HT causes in increase in the levels of inositol trisphosphate (InsP3), inositol bisphosphate (InsP2) and inositol phosphate (InsP1). InsP3 production was rapid and transient whereas InsP1 accumulated in a time and concentration dependent manner. The 5-HT stimulated InsP1 accumulation (pEC50 = 6.48) was potently and competitively inhibited by the 5-HT2 specific antag..

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