Journal article
5-HT1D receptors in guinea-pig and pigeon brain: Radioligand binding and biochemical studies
C Waeber, P Schoeffter, JM Palacios, D Hoyer
Naunyn Schmiedeberg S Archives of Pharmacology | SPRINGER | Published : 1989
DOI: 10.1007/BF00260601
Abstract
The characteristics of high affinity [3H]5-HT (5-hydroxytryptamine) binding to non 5-HTIA non 5-HT1A sites were examined in crude membranes prepared from different regions of guinea-pig and pigeon brains. The coupling of these sites to adenylate cyclase was examined, and its pharmacological profile investigated. In the presence of 100 nmol/1 8-OH-DPAT (8-hydroxy-2-(di-n-propylamino)tetralin) and 100 nmol/l mesulergine, [3H]5-HT labelled with nanomolar affinity an apparently homogeneous population of recognition sites in guinea-pig and pigeon brain membranes. The rank order of affinities of agonists and antagonists (5-CT (5-carboxamidotryptamine) > 5-HT > RU 24969 pyridinyl)-1H indole succina..
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