Journal article
Loss of Circulating CD4 T Cells with B Cell Helper Function during Chronic HIV Infection
KL Boswell, R Paris, E Boritz, D Ambrozak, T Yamamoto, S Darko, K Wloka, A Wheatley, S Narpala, A McDermott, M Roederer, R Haubrich, M Connors, J Ake, DC Douek, J Kim, C Petrovas, RA Koup
Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2014
Abstract
The interaction between follicular T helper cells (TFH) and B cells in the lymph nodes and spleen has a major impact on the development of antigen-specific B cell responses during infection or vaccination. Recent studies described a functional equivalent of these cells among circulating CD4 T cells, referred to as peripheral TFH cells. Here, we characterize the phenotype and in vitro B cell helper activity of peripheral TFH populations, as well as the effect of HIV infection on these populations. In co-culture experiments we confirmed CXCR5+ cells from HIV-uninfected donors provide help to B cells and more specifically, we identified a CCR7highCXCR5highCCR6highPD-1high CD4 T cell population ..
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Awarded by National Institute of Allergy and Infectious Diseases
Funding Acknowledgements
This research was supported by the Intramural Research Program of the Vaccine Research Center, NIAID, National Institutes of Health (AI064086; RH) and CAVD grant #OPP1032325 from the Bill and Melinda Gates Foundation (RAK), the University of California San Diego Center for AIDS Research (AI36214; RH) and the San Diego AIDS Clinical Trial Group (CTU AI69432; RH). The Children's National Medical Center (CNMC) under the auspices of the Basic Science Core of the District of Columbia Developmental Center for AIDS Research was funded by a grant from NIAID, NIH (P30AI087714). The work was also supported in part by an Interagency Agreement Y1-AI-2642-12 between the U.S. Army Medical Research and Material Command (USAMRMC) and the National Institutes of Allergy and Infectious Diseases and by a cooperative agreement (W81XWH-07-2-0067) between the Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., and the U.S. Department of Defense (DOD). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The opinions herein are those of the authors and should not be construed as official or representing the views of the U.S. Department of Health and Human Services, National Institute for Allergy and Infectious Diseases, the Department of Defense, or the Department of the Army.