Journal article

EFFECT OF LOCAL APPLICATION OF VINBLASTINE OR COLCHICINE ON ACETYLCHOLINE ACCUMULATION IN RAT SCIATIC-NERVE

PO HEIWALL, NR SAUNDERS, A DAHLSTROM, J HAGGENDAL

Acta Physiologica | WILEY-BLACKWELL | Published : 1976

Abstract

The accumulation of acetylcholine (ACh) following nerve crush was inhibited by high concentration of colchicine (10(-1), 10(-2 M) and vinblastine (10(-2)-10(-4 M) in a dose-dependent manner. The two mitotic inhibitors were effective both when applied to axons (subepineural injections) and to perikaraya (injections into the lumbar intumescence). The results indicate that mitotic inhibitors, when applied locally to cholinergic motor neurons, may block the proximo-distal transport of ACh. The possibility is discussed that this blockade is caused by an interference with a microtubule mechanism in the axons. Evidence for an increased local synthesis of ACh near the site of injection into nerves a..

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