ANGIOTENSIN II RECEPTOR SUBTYPES IN RAT BRAIN

Abstract

1. Angiotensin II (AII) receptor binding was localized in the rat brain by in vitro autoradiography using the antagonist analogue, 125I‐[Sar1, Ile8] AII. AII receptor binding was then differentiated into type I and type II subtypes by displacement with unlabelled non‐peptide antagonists specific for AII subtypes. 2. Type I binding was determined as that inhibited by Dup753 (10 μmol/L) and type II binding as that inhibited by XD329‐1 (10 μmol/L). The reducing agent dithiothreitol (DTT) decreased the binding to type I receptors and enhanced the binding to type II receptors. 3. Structures such as the vascular organ of the lamina terminalis, subfornical organ, median preoptic nucleus, area postr..