Journal article

CONCURRENT PRODUCTION OF INTERLEUKIN-2, INTERLEUKIN-10, AND GAMMA-INTERFERON IN THE REGIONAL LYMPH-NODES OF MICE WITH INFLUENZA PNEUMONIA

SR SARAWAR, PC DOHERTY

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 1994

Abstract

Cytokine production has been assessed at the single-cell level (ELISPOT assay) for freshly isolated mediastinal lymph node cells from C57BL/6 mice with primary, nonfatal influenza pneumonia. The mediastinal lymph node populations were also secondarily stimulated in vitro, and culture supernatants were assayed by enzyme-linked immunosorbent assay. Both approaches showed minimal evidence of protein secretion for interleukin-4 (IL-4), IL-5, and tumor necrosis factor, while IL-2, IL-10, and gamma interferon (IFN-gamma) were prominent throughout the response. The numbers of IL-2- and IFN-gamma-producing cells were maximal at 7 days after infection, while the total counts for cells secreting IL-10..

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