Limit-dilution analysis of weak influenza-immune T responses associated with H-2K(b) and H-2D(b1)

Abstract

A limiting-dilution analysis has been made of influenza-immune cytotoxic T lymphocyte (CTL) response patterns associated with H-2K(b) in and H-2D(d) in the B10.A(5R) and H-2K(d) and H-2D(b) in the B10.A(2R) and B10.A(4R) recombinant mouse strains. In previous in vivo experiments the H-2K(b) allele has been shown to be associated with low responsiveness or nonresponsiveness, whereas D(b)-restricted influenza-specific responses were found to be weak in recombinant strains that possessed the K(k) allele. Influenza-immune CTL restricted to the more 'dominant' H-2D(b) and H-2K(k) alleles were found consistently when primed T cells were restimulated in vitro under limiting-dilution conditions. Pre..