NEGATIVELY SELECTED H-2BML AND H-2B T-CELLS STIMULATED WITH VACCINIA VIRUS COMPLETELY DISCRIMINATE BETWEEN MUTANT AND WILD-TYPE H-2K ALLELES
PC DOHERTY, JR BENNINK, PJ WETTSTEIN
JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 1981
Lymphocyte populations from B6, C-H-2bml (KbmlDb) mutant mice cannot, after both in vivo and in vitro negative selection for alloreactivity, be induced to recognize vaccinia virus presented in the context of H-2Kb. This finding may mean that the T cell receptor(s) expresses a component that is very specific for a particular "active site" on the self-H-2 molecule. Alternatively, (if recognition is directed at a virus-H-2 complex) the more similar 2 H-2 molecules are, the more likely it may be that precursor thymocytes in the mutant with the capacity to bind H-2Kb + vaccinia virus may be deleted during ontogeny as a result of cross-reaction with H-2Kbml + endogenous antigen.