Cytotoxic T cell response to lymphocytic choriomeningitis virus. Properties of precursors of effector T cells, primary effector T cells and memory T cells in vitro and in vivo

Abstract

Spleen cells from CBA/H mice preprimed i.v. at various intervals with lymphocytic choriomeningitis (LCM) virus were tested for their capacity to respond and generate cytotoxic effector T cells on secondary stimulation both in vitro and in vivo. In vitro secondary stimulation was performed by culturing preprimed spleen cells with infected, syngeneic, peritoneal 'stimulator' cells for periods of up to 7 days at 37°. Controls were incubated with uninfected 'stimulators'. In vivo secondary stimulation was obtained by i.v. transfer of preprimed spleen cells into irradiated, heavily infected CBA/H recipients at various times prior to assay of recipients' spleens. Controls were uninfected, irradiat..