Journal article

ANTIBODIES ELICITED BY INFLUENZA-VIRUS HEMAGGLUTININ FAIL TO BIND TO SYNTHETIC PEPTIDES REPRESENTING PUTATIVE ANTIGENIC SITES

A NESTOROWICZ, GW TREGEAR, CN SOUTHWELL, J MARTYN, JM MURRAY, DO WHITE, DC JACKSON

MOLECULAR IMMUNOLOGY | PERGAMON-ELSEVIER SCIENCE LTD | Published : 1985

Abstract

A number of peptides of the hemagglutinin (HA) of X-31 influenza virus have been synthesised. The amino acid sequences of some of these peptides represent regions of HA which have been postulated [Wiley et al., Nature, Lond. 289, 373-378 (1981)] to form the antigenic sites of this molecule. Animals were immunized with free peptide or peptide conjugated to a carrier and the resulting antisera examined for their capacities to bind to homologous peptide, whole HA, reduced and alkylated HA, and intact virus. Not all peptides examined in this way were immunogenic. Only antibodies raised against the C-terminus of HA1 peptide displayed binding to virus. This antiserum bound to the intact HA but not..

View full abstract

University of Melbourne Researchers