Journal article

DETECTION AND ISOLATION OF AN IA GLYCOPROTEIN ANTIGEN FROM MOUSE SERUM

DC JACKSON, CR PARISH, IFC MCKENZIE

IMMUNOGENETICS | SPRINGER VERLAG | Published : 1977

Abstract

Ia antigenic activity is present in mouse serum and exists predominantly (>90 percent) as a dialyzable oligosaccharide which can be purified readily. This paper describes experiments which indicate, however, that a small proportion (<10 percent) of the Ia antigenic activity in serum, particularly from mitogen-injected mice, exists in a high molecular-weight form. Furthermore, this high molecular-weight Ia antigen, like the Ia oligosaccharide, appears to be secreted by T lymphocytes, particularly by T cells which have been activated recently by mitogens. The high molecular-weight Ia antigen was isolated from mouse serum by a procedure which utilized salt fractionation, gel filtration, density..

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University of Melbourne Researchers