Changes in the Surface of Virus‐Infected Cells Recognized by Cytotoxic T Cells: I. Minimal Requirements for Lysis of Ectromelia‐Infected P‐815 Cells

Abstract

P‐815 mastocytoma cells develop susceptibility to immune T‐cell‐mediated cytolysis shortly after infection by ectromelia virus. Intracellular viral replication and late protein synthesis seem to be unnecessary events. Interference with early protein synthesis, however, inhibits the development of susceptibility to lysis. The important intracellular events necessary for subsequent cytolysis appear to occur within 1 hr of infection. Virus rendered noninfectious by ultraviolet irradiation but not by γ irradiation is able to induce these changes. By determining the minimum and essential events of the infectious process which result in T‐cell‐mediated cytolysis, the task of establishing the molec..