Changes in the Surface of Virus‐Infected Cells Recognized by Cytotoxic T Cells: II. A Requirement for Glycoprotein Synthesis in Virus‐Infected Target Cells

Abstract

Infection of cells with either ectromelia or lymphocytic choriomeningitis (LCM) virus in the presence of 2‐deoxy‐D‐glucose (2‐DOG) Inhibited by up to 70% the extent to which the infected cells become susceptible to virus‐specific cell‐mediated lysis. The concentration of 2‐DOG used had little effect on the extent of total protein synthesis (incorporation of [35S]methionine) but inhibited (up to 25%) glycoprotein synthesis, as measured by incorporation of [3H]fucose. This suggested that glycoprotein synthesis was a necessary event for infected cells to become susceptible to T‐cell‐mediated lysis. The profiles (polyacrylamide gel electrophoresis) of newly synthesized, cellular glycoproteins fr..