Journal article
Immortalized Parkinson's disease lymphocytes have enhanced mitochondrial respiratory activity
SJ Annesley, ST Lay, SW De Piazza, O Sanislav, E Hammersley, CY Allan, LM Francione, MQ Bui, ZP Chen, KRW Ngoei, F Tassone, BE Kemp, E Storey, A Evans, DZ Loesch, PR Fisher
Dmm Disease Models and Mechanisms | COMPANY BIOLOGISTS LTD | Published : 2016
DOI: 10.1242/dmm.025684
Abstract
In combination with studies of post-mortem Parkinson's disease (PD) brains, pharmacological and genetic models of PD have suggested that two fundamental interacting cellular processes are impaired-proteostasis and mitochondrial respiration. We have re-examined the role of mitochondrial dysfunction in lymphoblasts isolated from individuals with idiopathic PD and an age-matched control group. As previously reported forvarious PD cell types, the production of reactive oxygen species (ROS) by PD lymphoblasts was significantly elevated. However, this was not due to an impairment of mitochondrial respiration, as is often assumed. Instead, basal mitochondrial respiration and ATP synthesis are drama..
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Awarded by National Institute of Child Health and Human Development
Funding Acknowledgements
This study was supported by research funds from La Trobe University's Department of Microbiology (P.R.F.), School of Life Sciences (P.R.F.) and 'Understanding Diseases' Research Focus Area (A.J.S., P.R.F., D.L.); the National Institute of Child Health and Human Development [grant R01 HD 36071 to D.L.]; Michael J. Fox Foundation for Parkinson's Research and Shake It Up Australia Foundation [grant 10862 to P.R.F., S.J.A., D.J.]; the Australian National Health and Medical Research Council [grant 1068798 and fellowship 1078752 to B.E.K.) and the Australian Research Council [grant DP130104548 to B.E.K.]. This work was supported in part by the Department of Health, State Government of Victoria Operational Infrastructure Support Program.