Journal article

ER-MP12 ANTIGEN, A NEW CELL-SURFACE MARKER ON MOUSE BONE-MARROW CELLS WITH THYMUS-REPOPULATING ABILITY .2. THYMUS-HOMING ABILITY AND PHENOTYPIC CHARACTERIZATION OF ER-MP12-POSITIVE BONE-MARROW CELLS

WAT SLIEKER, JCM VANDERLOO, MFTR DERIJKDEBRUIJN, DI GODFREY, PJM LEENEN, W VANEWIJK

INTERNATIONAL IMMUNOLOGY | OXFORD UNIV PRESS UNITED KINGDOM | Published : 1993

Abstract

In the accompanying paper we showed that six distinct subsets of bone marrow (BM) cells can be identified using the mAb ER-MP12 and ER-MP20 in two-colour immunofluorescence analysis. Upon intrathymic transfer into sublethally irradiated mice thymus-repopulating ability was restricted to ER-MP20- BM cells expressing either high or intermediate levels of the ER-MP12 antigen (1-2% and approximately 30% of BM nucleated cells respectively). The highest frequency of thymus-repopulating cells was found in the minor subset of ER-MP12(+)+20- BM cells. In the present study we demonstrate that upon intravenous transfer, thymus-homing and -repopulating BM cells are exclusively confined to the ER-MP12(+)..

View full abstract

University of Melbourne Researchers