Journal article
Novel metastatic models of esophageal adenocarcinoma derived from FLO-1 cells highlight the importance of E-cadherin in cancer metastasis
DS Liu, SJM Hoefnagel, OM Fisher, KK Krishnadath, KG Montgomery, RA Busuttil, AJ Colebatch, M Read, CP Duong, WA Phillips, NJ Clemons
Oncotarget | IMPACT JOURNALS LLC | Published : 2016
Abstract
There is currently a paucity of preclinical models available to study the metastatic process in esophageal cancer. Here we report FLO-1, and its isogenic derivative FLO-1LM, as two spontaneously metastatic cell line models of human esophageal adenocarcinoma. We show that FLO-1 has undergone epithelial-mesenchymal transition and metastasizes following subcutaneous injection in mice. FLO-1LM, derived from a FLO-1 liver metastasis, has markedly enhanced proliferative, clonogenic, anti-apoptotic, invasive, immune-tolerant and metastatic potential. Genome-wide RNAseq profiling revealed a significant enrichment of metastasis-related pathways in FLO-1LM cells. Moreover, CDH1, which encodes the adhe..
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Funding Acknowledgements
This work was supported by a National Health and Medical Research Council (NHMRC) of Australia Centres for Research Excellence grant (WAP) and a Peter MacCallum Cancer Foundation New Investigator grant (DSL); Royal Australasian College of Surgeons (RACS) Foundation for Surgery John Loewenthal, Reg Worcester and Eric Bishop Fellowships, Cancer Therapeutics Scholarship (Cancer Therapeutics CRC) and NHMRC Postgraduate Research Scholarship to DSL; NHMRC Postgraduate Research Scholarship and Swiss National Science Foundation Doc.Mobility Grant to OMF; RACS Thornell-Shore Memorial Scholarship and Melbourne University Sir Thomas Naghten Fitzgerald Scholarship to MR.