Journal article

Optimal Periprosthetic Tissue Specimen Number for Diagnosis of Prosthetic Joint Infection

Trisha N Peel, Tim Spelman, Brenda L Dylla, John G Hughes, Kerryl E Greenwood-Quaintance, Allen C Cheng, Jayawant N Mandrekar, Robin Patel

Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 2017

Abstract

We recently demonstrated improved sensitivity of prosthetic joint infection (PJI) diagnosis using an automated blood culture bottle system for periprosthetic tissue culture [T. N. Peel et al., mBio 7(1):e01776-15, 2016, https://doi.org/10.1128/mBio.01776-15]. This study builds on the prior research by examining the optimal number of periprosthetic tissue specimens required for accurate PJI diagnosis. Current guidelines recommend five to six, which is impractical. We applied Bayesian latent class modeling techniques for estimating diagnostic test properties of conventional culture techniques (aerobic and anaerobic agars and thioglycolate broth) compared to inoculation into blood culture bottl..

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Grants

Awarded by National Health and Medical Research Council Medical Early Career Fellowship


Awarded by National Health and Medical Research Council Career Development Fellowship 2


Awarded by National Institutes of Health


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Awarded by NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES


Funding Acknowledgements

R.P. reports grants from BioFire, Check-Points, Curetis, 3M, Merck, Hutchison Biofilm Medical Solutions, Accelerate Diagnostics, Allergan, and The Medicines Company. R.P. is a consultant to Curetis, Roche, Qvella, and Diaxonhit. In addition, R.P. has a patent on a Bordetella pertussis/B. parapertussis PCR assay with royalties paid by TIB, a patent on a device/method for sonication with royalties paid by Samsung to Mayo Clinic, and a patent on an antibiofilm substance issued. R.P. serves on an Actelion data monitoring board. R.P. receives travel reimbursement and an editor's stipend from ASM and IDSA and honoraria from the USMLE, Up-to-Date, and the Infectious Diseases Board Review Course.Trisha N. Peel was supported by the Richard Memorial Kemp Fellowship, Royal Australasian College of Physicians, and the National Health and Medical Research Council Medical Early Career Fellowship (APP1069734). Allen C. Cheng was supported by a National Health and Medical Research Council Career Development Fellowship 2 (APP1068732). Robin Patel was supported by the National Institutes of Health (R01 AR56647 and R01 AI91594).