Journal article
KNS4/UPEX1: A Type II Arabinogalactan beta-(1,3)-Galactosyltransferase Required for Pollen Exine Development
Toshiya Suzuki, Joan Onate Narciso, Wei Zeng, Allison van de Meene, Masayuki Yasutomi, Shunsuke Takemura, Edwin R Lampugnani, Monika S Doblin, Antony Bacic, Sumie Ishiguro
Plant Physiology | American Society of Plant Biologists | Published : 2017
DOI: 10.1104/pp.16.01385
Open access
Abstract
Pollen exine is essential for protection from the environment of the male gametes of seed-producing plants, but its assembly and composition remain poorly understood. We previously characterized Arabidopsis (Arabidopsis thaliana) mutants with abnormal pollen exine structure and morphology that we named kaonashi (kns). Here we describe the identification of the causal gene of kns4 that was found to be a member of the CAZy glycosyltransferase 31 gene family, identical to UNEVEN PATTERN OF EXINE1, and the biochemical characterization of the encoded protein. The characteristic exine phenotype in the kns4 mutant is related to an abnormality of the primexine matrix laid on the surface of developin..
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Awarded by KAKENHI
Awarded by Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science (MEXT/JSPS), Japan
Awarded by Australia Research Council (ARC)
Awarded by Grants-in-Aid for Scientific Research
Funding Acknowledgements
This work was supported by KAKENHI grant no. 20000304 (to T.S.); grants no. 21024004, 23370018, and 15H01229 (to S.I.) from the Ministry of Education, Culture, Sports, Science and Technology/Japan Society for the Promotion of Science (MEXT/JSPS), Japan; grant no. CE1101007 (to W.Z., E.R.L., A.v.d.M., M.S.D., and A.B.) from the Australia Research Council (ARC) to the ARC Centre of Excellence in Plant Cell Walls; and the University of Melbourne under the Melbourne International Research Scholarship (MRS) and Melbourne International Fee Remission Scholarship (MIFRS) (to J.O.N.).