Journal article

The complexities and caveats of lineage tracing in the mammary gland

Anne C Rios, Nai Yang Fu, Joseph Cursons, Geoffrey J Lindeman, Jane E Visvader

BREAST CANCER RESEARCH | BIOMED CENTRAL LTD | Published : 2016

Abstract

Lineage tracing is increasingly being utilised to probe different cell types that exist within the mammary gland. Whilst this technique is powerful for tracking cells in vivo and dissecting the roles of different cellular subsets in development, homeostasis and oncogenesis, there are important caveats associated with lineage tracing strategies. Here we highlight key parameters of particular relevance for the mammary gland. These include tissue preparation for whole-mount imaging, whereby the inclusion of enzymatic digestion can drastically alter tissue architecture and cell morphology, and therefore should be avoided. Other factors include the scoring of clones in three dimensions versus two..

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Grants

Awarded by ARC


Funding Acknowledgements

We are grateful to Gordon Smyth for discussions on statistics. All mouse experiments were approved by The WEHI Animal Ethics Committee. This work was supported by the Australian National Health and Medical Research Council (NHMRC). ACR and NYF were supported by a National Breast Cancer Foundation Fellowship, JC by the ARC (CE140100036), GJL by a NHMRC Research Fellowship, and JEV by an Australia Fellowship.