Journal article

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

Frisca Frisca, Daniel Colquhoun, Yona Goldshmit, Minna-Liisa Anko, Alice Pebay, Jan Kaslin



Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer's vesicle (KV..

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Awarded by NHMRC

Awarded by Australian Research Council (ARC)

Funding Acknowledgements

The authors would like to thank Karuna Sampath for providing the spaw and lefty2 constructs, Brian Ciruna for providing the arl13b-GFP construct, Peter D Currie for providing the gsc construct, and Lee Miles for providing the ntl construct. This work was supported by an NHMRC project grant (GNT1068411), Monash University Faculty of Medicine and Nursing strategic grant and Operational Infrastructure Support from the Victorian Government. J.K. is supported by an Eva and Les Erdi fellowship. F.F. received an Australia Awards by the Australian government (AusAID). A.P. is supported by an Australian Research Council (ARC, FT140100047) Future Fellowship. We thank Monash Medical Micro Imaging Facility and Fish Core for excellent support.