Journal article

Molecular characterisation of Theileria orientalis in imported and native bovines from Pakistan

Hagos Gebrekidan, Tariq Abbas, Muhammad Wajid, Aamir Ali, Robin B Gasser, Abdul Jabbar

INFECTION GENETICS AND EVOLUTION | ELSEVIER | Published : 2017

Abstract

The epidemiological aspects of Theileria orientalis in Pakistan are unknown; therefore, investigations using sensitive and precise molecular techniques are required. This study reports the first molecular characterisation of T. orientalis detected from imported (Bos taurus) and native cattle (Bos indicus×Bos taurus) and buffaloes (Bubalus bubalis) selected from four districts of Punjab, Pakistan. DNA samples from blood (n=246) were extracted and tested using conventional PCR utilising the major piroplasm surface protein (MPSP) gene and multiplexed tandem PCR (MT-PCR). Theileria orientalis DNA was detected (15%; 22/147) only in imported cattle by conventional PCR, whereas 24.5% (36/147), 6% (..

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