Molecular characterisation of Theileria orientalis in imported and native bovines from Pakistan
Hagos Gebrekidan, Tariq Abbas, Muhammad Wajid, Aamir Ali, Robin B Gasser, Abdul Jabbar
INFECTION GENETICS AND EVOLUTION | ELSEVIER | Published : 2017
The epidemiological aspects of Theileria orientalis in Pakistan are unknown; therefore, investigations using sensitive and precise molecular techniques are required. This study reports the first molecular characterisation of T. orientalis detected from imported (Bos taurus) and native cattle (Bos indicus×Bos taurus) and buffaloes (Bubalus bubalis) selected from four districts of Punjab, Pakistan. DNA samples from blood (n=246) were extracted and tested using conventional PCR utilising the major piroplasm surface protein (MPSP) gene and multiplexed tandem PCR (MT-PCR). Theileria orientalis DNA was detected (15%; 22/147) only in imported cattle by conventional PCR, whereas 24.5% (36/147), 6% (..View full abstract
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This project was supported by Collaborative Research and Early Career Researchers grants (the University of Melbourne) (A.J.). H.G. is a grateful recipient of the Melbourne International Research Scholarships (MIRS) and Melbourne International Fee Remission Scholarships (MIFRS) through The University of Melbourne. The dairy farmers' collaborations are appreciated.