The Type III Effector NleD from Enteropathogenic Escherichia coli Differentiates between Host Substrates p38 and JNK
Kristina Creuzburg, Cristina Giogha, Tania Wong Fok Lung, Nichollas E Scott, Sabrina Muehlen, Elizabeth L Hartland, Jaclyn S Pearson
INFECTION AND IMMUNITY | AMER SOC MICROBIOLOGY | Published : 2017
Enteropathogenic Escherichia coli (EPEC) is a gastrointestinal pathogen that utilizes a type III secretion system (T3SS) to inject an array of virulence effector proteins into host enterocytes to subvert numerous cellular processes for successful colonization and dissemination. The T3SS effector NleD is a 26-kDa zinc metalloprotease that is translocated into host enterocytes, where it directly cleaves and inactivates the mitogen-activated protein kinase signaling proteins JNK and p38. Here a library of 91 random transposon-based, in-frame, linker insertion mutants of NleD were tested for their ability to cleave JNK and p38 during transient transfection of cultured epithelial cells. Immunoblo..View full abstract
Awarded by Australian National Health and Medical Research Council (NHMRC)
This work was supported by grants to E.L.H. from the Australian National Health and Medical Research Council (NHMRC) (APP1100609). K.C. is supported by a Deutsche Forschungsgemeinschaft (DFG) research fellowship. J.S.P. is the recipient of an NHMRC early career research fellowship. C.G. is the recipient of an Australian Postgraduate Award (APA). T.W.F.L. is a recipient of a University of Melbourne International Research Scholarship (MIRS). N.E.S. is the recipient of an NHMRC early career research fellowship.