RETENTION OF INTACT HSA FOR PROLONGED PERIODS IN THE POPLITEAL LYMPH-NODES OF SPECIFICALLY IMMUNIZED MICE

Abstract

The rate of clearance of radiolabel was studied from the popliteal lymph nodes of either nonimmune or specifically immunized mice following footpad injection of 125I-labeled HSA. The 125I was more rapidly and more completely eliminated from the nodes of nonimmune mice than from the nodes of specifically immunized animals. Autoradiographs showed that, whereas the radiolabel was strictly confined for long periods to the follicles of the nodes of immune mice, no such localizaton was present in nonimmune animals. Solubilization of the whole nodes with 5 M guanidine-HCl released the labeled material which could then be specifically precipitated with anti-HSA but not with anti-EA. Such specificall..