BIOCHEMICAL AND FUNCTIONAL-CHARACTERIZATION OF MATURE PROGENY PURIFIED FROM A MYELOMONOCYTIC LEUKEMIA-CELL LINE

Abstract

Comparative studies were performed on the cloned myelomonocytic leukemia cell line, WEHI-3B, and a subcloned line, WEHI-3BM6. WEHI-3BM6 cells were less responsive than WEHI-3B cells to differentiation factor (DF) present in the sera of mice injected with endotoxin (endotoxin serum, ES). WEHI-3BM6 cells produced only 8% monocytes after 6 days of incubation with ES compared with 68% monocytes produced by WEHI-3B cells. In the presence of ES the rate of differentiation of both cell lines was enhanced by the addition of actinomycin D (5 ng/ml) such that after 2 days of stimulation 62% of the cells were mature monocytes. Lysozyme content as well as the expression of alpha-napthyl acetate esterase..