Surface Antigens on Normal and Leukaemic Human Cells Detected by Monoclonal Antibodies

Abstract

Surface antigens were analyzed on normal human marrow and chronic myeloid leukaemic cells using 4 monoclonal mouse anti‐human antibodies. The fluorescence‐activated cell sorter was used to quantify the binding of each antibody to different subpopulations of cells, and sorted fractions were cultured in agar‐medium to assay for granulocyte‐macrophage and eosinophil precursors. All cells in the granulocyte series including colony‐forming cells bound a similar quantity of an antibody to the human leucocyte common antigen. This antibody did not bind to cells in the erythroid series. A monoclonal antibody to antigen present on brain, lymphocytes and granulocytes (and almost certainly homologous to..