Production and characterization of monoclonal antibodies reactive with subsets of epidermal cells.
ID Gardner, RH Whitehead, AW Burgess
Pathology | Published : 1986
Monoclonal antibodies were raised against an extract of human callus to provide markers of cell differentiation. Three antibodies, LMM1, LMM2 and LMM3, have been isolated and react with different histological structures in skin. LMM1 reacts with two distinct proteins of 62-65 kD molecular weight and stains cells in the stratum spinosum and stratum granulosum of normal epidermis. This antibody stains a high proportion of cultured epidermal cells showing a diffuse cytoplasmic reactivity with nucleolar staining also being present in some cells. LMM2 reacts with several lower molecular weight proteins, the two major bands being 38 kD and 44 kD. It stains basal epidermal cells strongly and stratu..View full abstract