Separation of mouse bone marrow cells using wheat germ agglutinin affinity chromatographyy.
NA Nicola, AW Burgess, D Metcalf, FL Battye
Aust J Exp Biol Med Sci | Published : 1978
Mouse bone marrow cells were fractionated on columns of wheat germ agglutinin-Sepharose 6MB (WGA-Sepharose) and conditions established for specific binding and cell enrichment in the eluted fractions. A small proportion (7%) of the cells applied did not bind to the column and 60% of these were lymphocyte-like cells. Twice as many cells were eluted from the WGA-Sepharose column using N-acetyl-D-glucosamine (GlcNAc), and 80% of these cells were polymorphonuclear granulocytes and metamyelocytes. These cells were only released from the matrix in the presence of GlcNAc at high buffer flow rates (greater than 4 ml/min). Approximately 3 x 10(8) bone marrow cells bound to 1 ml of WGA-Sepharose. At l..View full abstract