Journal article

INVITRO ACTIONS ON HEMATOPOIETIC-CELLS OF RECOMBINANT MURINE GM-CSF PURIFIED AFTER PRODUCTION IN ESCHERICHIA-COLI - COMPARISON WITH PURIFIED NATIVE GM-CSF

D METCALF, AW BURGESS, GR JOHNSON, NA NICOLA, EC NICE, J DELAMARTER, DR THATCHER, JJ MERMOD

JOURNAL OF CELLULAR PHYSIOLOGY | WILEY-LISS | Published : 1986

Abstract

Recombinant murine GM-CSF produced in Escherichia coli was purified to homogeneity and tested in parallel with purified native GM-CSF. Both recombinant and native GM-CSF stimulated granulocyte and/or macrophage colony formation by adult and fetal mouse progenitor cells, and with adult marrow cells the specific activity of the recombinant GM-CSF (25 X 10(8) U/mg) was similar to that of the native form (15 X 10(8) U/mg). At high concentrations (greater than 200 U/ml), both forms of GM-CSF also stimulated eosinophil colony formation by adult marrow cells and, at very high concentrations (greater than 800 U/ml), megakaryocyte and some erythroid and mixed-erythroid colony formation. Recombinant G..

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