The effect of colony stimulating factor on the synthesis of ribonucleic acid by mouse bone marrow cells in vitro

Abstract

The effect of granulocyte‐macrophage colony stimulating factor (GM‐CSF) on the synthesis of RNA in liquid cultures of mouse bone marrow, spleen, thymus, peritoneal, peripheral blood leukocytes and lymph node cells was investigated. GM‐CSF appeared to stimulate RNA‐synthesis in syngeneic bone marrow cells within ten minutes of adding it to the culture. In the presence of GM‐CSF bone marrow cultures maintained their initial rate of RNA synthesis for approximately ten hours. GM‐CSF had no apparent effect on the uptake of 3H‐uridine into bone marrow cells. This stimulation was still observed in the presence of puromycin and cycloheximide, but was abrogated by actinomycin D. The magnitude of the ..