Granulocyte macrophage‐colony stimulating factor stimulates the synthesis of membrane and nuclear proteins in murine neutrophils

Abstract

The effect of granulocyte macrophage‐colony stimulating factor (GM‐CSF), a well‐characterized hemopoietic regulator, on protein synthesis in murine bone marrow neutrophils is described. Bone marrow neutrophils in excess of 95% purity were obtained by fluorescence‐activated cell sorting.While GM‐CSF did not appear to slow the rate of dying of peritoneal exudate neutrophils or thymus cells, the viability of bone marrow neutrophils after 17 hr was enhanced (40%) by GM‐CSF. GM‐CFS had no effect on total 35S‐methionine incorporation by thymocytcs or peritoneal exudate neutrophils over a 17‐hr incubation period; however, bone marrow neutrophils showed increased incorporation (approximately 10%) at..