Journal article
Construction, production, and characterization of humanized anti-Lewis Y monoclonal antibody 3S193 for targeted immunotherapy of solid tumors
AM Scott, D Geleick, M Rubira, K Clarke, EC Nice, FE Smyth, E Stockert, EC Richards, FJ Carr, WJ Harris, KL Armour, J Rood, A Kypridis, V Kronina, R Murphy, FT Lee, Z Liu, K Kitamura, G Ritter, K Laughton Show all
Cancer Research | AMER ASSOC CANCER RESEARCH | Published : 2000
Abstract
The Lewis Y (Le(y)) antigen is a blood group-related antigen that is expressed in a high proportion of epithelial cancers (including breast, colon, ovary, and lung cancer) and is an attractive target for monoclonal antibody-directed therapy. The murine monoclonal 3S193 (IgG3) was generated in BALB/c mice by immunization with Le(y)-expressing cells of the MCF-7 breast carcinoma cell-line. The murine 3S193 showed high specificity for Le(y) in ELISA tests with synthetic Le(y) and Le(y)-containing glycoproteins and glycolipids and also reacted strongly in rosetting assays and cytotoxic tests with Le(y)-expressing cells. We generated a humanized form of the murine 3S193 antibody by linking cDNA s..
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