Journal article

Multimodal vibrational imaging of cells

David Perez-Guaita, Kamila Kochan, Miguela Martin, Dean W Andrew, Philip Heraud, Jack S Richards, Sayden R Wood

VIBRATIONAL SPECTROSCOPY | ELSEVIER SCIENCE BV | Published : 2017

Abstract

Fourier Transform Infrared (FTIR) and Raman imaging offer complementary information about the spatial location of molecules within cells. In this paper we investigate the integration of both imaging modalities in an extended image containing unique FTIR and Raman spectra for each pixel. Two types of cells were investigated: red blood cells infected with the Plasmodium falciparum parasite and Micrasterias, a desmid microalgal species. The microscope configuration and pixel size were selected specifically for acquiring images with the same pixel size, and samples were fixed to a sample holder in order to measure the same cells with the same orientation. Images were first analysed individually ..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Discovery Future Fellowship grant


Awarded by Australian Research Council Discovery Grant


Awarded by Polish National Science Centre


Funding Acknowledgements

B. R. Wood is supported by an Australian Research Discovery Future Fellowship grant FT120100926. Funding to the authors was also provided by the Australian Research Council Discovery Grant DP140102504 and the National Health and Medical Research Council of Australia (Early Career Fellowships to J. Richards; Infrastructure for Research Institutes Support Scheme Grant to the Burnet Institute); Victorian State Government Operational Infrastructure Support Grant to the Burnet Institute. KK received funds from Polish National Science Centre in the form of financing of a scholarship based on the decision No DEC-2015/16/T/NZ7/00155. We also acknowledge Mr Finlay Shanks for instrumental support.