Journal article

THYMIC MICROENVIRONMENTAL ABNORMALITIES AND THYMIC SELECTION IN NZB.H-2(BM12) MICE

Y WATANABE, M NAIKI, T WILSON, D GODFREY, BL CHIANG, R BOYD, A ANSARI, ME GERSHWIN

JOURNAL OF IMMUNOLOGY | AMER ASSOC IMMUNOLOGISTS | Published : 1993

Abstract

We have taken advantage of an extensive panel of mAb directed to thymic epithelial and nonepithelial stromal cells to examine the expression of these Ag from day 16 of gestation through 6 mo of age in NZB(H-2d), NZB.H-2b, NZB.H-2bm12, C57BL/6(H-2b), and C57BL/6.H-2bm12 mice. In addition, by triple color flow cytometry we have examined the expression of cell surface markers defining distinct stages of intrathymic T cell maturation. New Zealand mice demonstrated three abnormalities. MTS 10 normally stains thymic medullary and subcapsular epithelium. However, New Zealand mice demonstrated striking irregular medullary epithelial cell shape whereas their subcapsular epithelium remained normal. Mo..

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University of Melbourne Researchers