Journal article

SIMILAR PEPTIDES FROM 2 BETA-CELL AUTOANTIGENS, PROINSULIN AND GLUTAMIC-ACID DECARBOXYLASE, STIMULATE T-CELLS OF INDIVIDUALS AT RISK FOR INSULIN-DEPENDENT DIABETES

G RUDY, N STONE, LC HARRISON, PG COLMAN, P MCNAIR, V BRUSIC, MB FRENCH, MC HONEYMAN, B TAIT, AM LEW

Molecular Medicine | BLACKWELL SCIENCE PUBL INC CAMBRIDGE | Published : 1995

Abstract

BACKGROUND: Insulin (1) and glutamic acid decarboxylase (GAD) (2) are both autoantigens in insulin-dependent diabetes mellitus (IDDM), but no molecular mechanism has been proposed for their association. We have identified a 13 amino acid peptide of proinsulin (amino acids 24-36) that bears marked similarity to a peptide of GAD65 (amino acids 506-518) (G. Rudy, unpublished). In order to test the hypothesis that this region of similarity is implicated in the pathogenesis of IDDM, we assayed T cell reactivity to these two peptides in subjects at risk for IDDM. MATERIALS AND METHODS: Subjects at risk for IDDM were islet cell antibody (ICA)-positive, first degree relatives of people with insulin-..

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