Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells
Robert Mahen, Birgit Koch, Malte Wachsmuth, Antonio Z Politi, Alexis Perez-Gonzalez, Julia Mergenthaler, Yin Cai, Jan Ellenberg
Molecular Biology of the Cell | AMER SOC CELL BIOLOGY | Published : 2014
Awarded by European Commission within the MitoSys and SystemsMicroscopy consortia
Awarded by EMBO fellowship
Awarded by Wellcome Trust Henry Wellcome Fellowship
We thank Tony Hyman and Ina Poser for donating the mouse BAC AURKB-GFP and thank all members of the Ellenberg laboratory for support. We thank Kota Miura for help in setting up the automated Fiji analysis and Bianca Nijmeijer for help with Southern blotting. This study was supported by funding from the European Commission within the MitoSys and SystemsMicroscopy consortia (FP7/2007-2013-241548 and FP7/2007-2013-258068). R. M. was supported by an EMBO fellowship (ALTF 416-2012, GA-2010-267146) and a Wellcome Trust Henry Wellcome Fellowship (100090/Z/12/Z).