Journal article
Role of lipids in killing mycobacteria by macrophages: Evidence for NF-κB-dependent and -independent killing induced by different lipids
MG Gutierrez, AP Gonzalez, E Anes, G Griffiths
Cellular Microbiology | WILEY | Published : 2009
Abstract
We have shown that several lipids can modulate the macrophage innate immune response against mycobacteria and enhance their killing. Since NF-κB is required for mycobacterial killing, we tested the ability of lipids to activate NF-κB in uninfected macrophages and those infected with mycobacteria. In uninfected cells, sphingomyelin (SM), phosphatidylinositol-4-phosphate (PIP) and arachidonic acid (AA) enhanced NF-κB activation and the cell surface expression of CD69, a macrophage activation marker regulated by NF-κB. Sphingosine (Sph), sphingosine-1-phosphate (S1P), diacylglycerol (DAG), eicosapentanoic acid (EPA) and phosphatidyl choline (PC) failed to activate either NF-κB or CD69. Ceramide..
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Awarded by DFG
Awarded by Fundacao para a Ciencia e a tecnologia (FCT)
Funding Acknowledgements
We are grateful to David Liebl for critical reading of this manuscript. We would also like to thank Stefan Terjung at the Advanced Light Microscopy Facility and Andy Riddell at the Flow Cytometry Facility (EMBL, Heidelberg) for excellent technical assistance and advice. M.G.G. was supported by a Research Fellowship from the Alexander von Humboldt Foundation and is currently funded by an EMBO Fellowship. G.G. was partially supported by DFG Sphingolipid Network Grant SPP-1267. E.A. was supported by Fundacao para a Ciencia e a tecnologia (FCT) Grant PPCDT/BIA-BCM/55327/2007.