Journal article
Establishment of memory CD8 T cells with live attenuated influenza virus across different vaccination doses
Z Wang, L Kedzierski, S Nuessing, BYL Chua, SM Quiñones-Parra, VC Huber, DC Jackson, PG Thomas, K Kedzierska
Journal of General Virology | MICROBIOLOGY SOC | Published : 2016
DOI: 10.1099/jgv.0.000651
Abstract
FluMist has been used in children and adults for more than 10 years. As pre-existing CD8+ T cell memory pools can provide heterologous immunity against distinct influenza viruses, it is important to understand influenza-specific CD8+ T cell responses elicited by different live attenuated influenza virus (LAIV) regimens. In this study, we immunized mice intranasally with two different doses of live-attenuated PR8 virus (PR8 ts, H1N1), low and high, and then assessed protective efficacy by challenging animals with heterosubtypic X31-H3N2 virus at 6 weeks postvaccination. Different LAIV doses elicited influenza-specific CD8+ T cell responses in lungs and spleen, but unexpectedly not in bronchoa..
View full abstractRelated Projects (6)
Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was supported by the Australian National Health and Medical Research Council (NHMRC) Program Grant to K. K. and D. C. J. (ID 1071916). K. K. is an NHMRC SRFB Fellow (ID 1102792) and D. C. J. is an NHMRC SPRF Fellow. S. N. is supported by a Melbourne International Fee Remission Scholarship (MIFRS) and Melbourne International Research Scholarship (MIRS).