Journal article
Set7 mediated interactions regulate transcriptional networks in embryonic stem cells
NK Tuano, J Okabe, M Ziemann, ME Cooper, A El-Osta
Nucleic Acids Research | OXFORD UNIV PRESS | Published : 2016
DOI: 10.1093/nar/gkw621
Open access
Abstract
Histone methylation by lysine methyltransferase enzymes regulate the expression of genes implicated in lineage specificity and cellular differentiation. While it is known that Set7 catalyzes mono-methylation of histone and non-histone proteins, the functional importance of this enzyme in stem cell differentiation remains poorly understood. We show Set7 expression is increased during mouse embryonic stem cell (mESC) differentiation and is regulated by the pluripotency factors, Oct4 and Sox2. Transcriptional network analyses reveal smooth muscle (SM) associated genes are subject to Set7-mediated regulation. Furthermore, pharmacological inhibition of Set7 activity confirms this regulation. We o..
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Awarded by Juvenile Diabetes Research Foundation International
Funding Acknowledgements
National Health and Medical Research Council [NHMRC GNT0526681, GNT1048377]; Juvenile Diabetes Research Foundation [JDRF 5-2008-298, 27-2012-451]; Diabetes Australia Research Trust [DART]; Victorian Government's Operational Infrastructure Support program [in part]. Funding for open access charge: Baker IDI Heart and Diabetes Institute.