Journal article

RAT CALVARIAL CELL-LINES IMMORTALIZED WITH SV40 LARGE T-ANTIGEN - CONSTITUTIVE AND RETINOIC ACID-INDUCIBLE EXPRESSION OF OSTEOBLASTIC FEATURES

JK HEATH, SB RODAN, KG YOON, GA RODAN

ENDOCRINOLOGY | OXFORD UNIV PRESS INC | Published : 1989

Abstract

Two new bone cell lines were established by immortalizing cells derived from embryonic rat calvariae with a recombinant retrovirus containing the cDNA for SV-40 large T antigen and the neomycin resistance gene. One cell line, RCT-1, isolated from early digest cells, a population which typically does not express osteoblastic features, displayed osteoblastic characteristics only after 3 days of treatment with 1 microM retinoic acid: alkaline phosphatase activity increased from 0.003 to 0.25 mumol/min.mg protein, the steady state level of type I procollagen mRNA increased 4-fold, and the cells acquired a PTH-stimulatable adenylate cyclase (EC50, 10 nM). mRNA for osteopontin, an abundant bone ma..

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