Journal article

A FACTOR SYNTHESIZED BY RABBIT PERIOSTEAL FIBROBLASTS STIMULATES BONE-RESORPTION AND COLLAGENASE PRODUCTION BY CONNECTIVE-TISSUE CELLS-INVITRO

JK HEATH, MC MEIKLE, SJ ATKINSON, JJ REYNOLDS

BIOCHIMICA ET BIOPHYSICA ACTA | ELSEVIER SCIENCE BV | Published : 1984

Abstract

Unstimulated monolayer cultures of confluent rabbit periosteal fibroblasts synthesize a factor that stimulates bone resorption in vitro. Furthermore it stimulates rabbit chondrocytes and mouse osteoblasts to synthesize collagenase. The factor has no effect on dead bone in culture, and its activity on live bone is mediated principally by osteoclasts, since it is 75% inhibited by salmon calcitonin. Characterization of the factor by gel filtration and isoelectric focusing indicates an Mr in the range 15000-25000 and a pI corresponding to approx. pH 4.7. These biological and physiochemical properties are similar to those reported for a factor released by peripheral blood monocytes. However, wher..

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