Journal article

Highly Sensitive Detection of Minimal Cardiac Ischemia using Positron Emission Tomography Imaging of Activated Platelets

Melanie Ziegler, Karen Alt, Brett M Paterson, Peter Kanellakis, Alex Bobik, Paul S Donnelly, Christoph E Hagemeyer, Karlheinz Peter

Scientific Reports | NATURE PUBLISHING GROUP | Published : 2016

Abstract

A reliable method for the diagnosis of minimal cardiac ischemia would meet a strong demand for the sensitive diagnosis of coronary artery disease in cardiac stress testing and risk stratification in patients with chest pain but unremarkable ECGs and biomarkers. We hypothesized that platelets accumulate early on in ischemic myocardium and a newly developed technology of non-invasive molecular PET imaging of activated platelets can thus detect minimal degrees of myocardial ischemia. To induce different degrees of minimal cardiac ischemia, the left anterior descending artery (LAD) was ligated for 10, 20 or 60 min. Mice were injected with a newly generated scFvanti-GPIIb/IIIa-64CuMeCOSar radiotr..

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Grants

Funding Acknowledgements

We acknowledge the Centre for PET at the Austin (Melbourne, Australia) for supply of <SUP>64</SUP>Cu. This research was undertaken using equipment provided by Monash Biomedical Imaging, Monash University as part of the Victorian Biomedical Imaging Capability (Victorian Government). This work was supported by the National Health and Medical Research Council, the Australian Research Council, the German Research Foundation, the National Heart Foundation, the Victorian Government's Operational Infrastructure Support Program, Victoria's Science Agenda Strategic Project Fund and the PET Solid Target Laboratory.