Development of an ovine efferent mammary lymphatic cannulation model with minimal tissue damage
Hung-Hsun Yen, Elizabeth Washington, Wayne Kimpton, Evan Hallein, Joanne Allen, Silk Yu Lin, Stuart Barber
BMC Veterinary Research | BIOMED CENTRAL LTD | Published : 2016
BACKGROUND: Two mammary lymphatic cannulation models in sheep have been described with minimal use in the past 50 years. The purpose of this study was to investigate a new surgical technique to allow long term monitoring of mammary lymph flow and composition from the mammary glands, with rapid ewe recovery and minimal complications post-surgery. RESULTS: We developed a modified methodology for cannulating the efferent mammary lymphatic from the mammary lymph node with minimum tissue damage. Compared to the previous models, our method required only a small incision on the aponeurosis of the external abdominal oblique muscles and thus reduced the difficulties in suturing the aponeurosis. It al..View full abstract
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This study was funded by an Early Career Researcher grant from the University of Melbourne and by Australian sheep producers and the Australian Government through Meat & Livestock Australia (MLA) Limited. Neither of these organisations were involved in the design, interpretation or reporting of study results.