Journal article
Mature IgM-expressing plasma cells sense antigen and develop competence for cytokine production upon antigenic challenge
P Blanc, L Moro-Sibilot, L Barthly, F Jagot, S This, S De Bernard, L Buffat, S Dussurgey, R Colisson, E Hobeika, T Fest, M Taillardet, O Thaunat, A Sicard, P Mondière, L Genestier, SL Nutt, T Defrance
Nature Communications | NATURE PUBLISHING GROUP | Published : 2016
DOI: 10.1038/ncomms13600
Open access
Abstract
Dogma holds that plasma cells, as opposed to B cells, cannot bind antigen because they have switched from expression of membrane-bound immunoglobulins (Ig) that constitute the B-cell receptor (BCR) to production of the secreted form of immunoglobulins. Here we compare the phenotypical and functional attributes of plasma cells generated by the T-cell-dependent and T-cell-independent forms of the hapten NP. We show that the nature of the secreted Ig isotype, rather than the chemical structure of the immunizing antigen, defines two functionally distinct populations of plasma cells. Fully mature IgM-expressing plasma cells resident in the bone marrow retain expression of a functional BCR, wherea..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
We would like to thank Professor Michael Reth (Centre for Biological Signalling Studies BIOSS, Freiburg, Germany) for the kind gift of the mEGFP/mb-1<SUP>inv</SUP> mice. We thank T. Andrieu (SFR BioSciences, UMS3444/US8) for technical assistance for flow cytometry and cell sorting; N Aguilera and J-F Henry (PBES, SFR BioSciences) for help at the animal care facility. Pascal Blanc was supported by a grant from Region Rhones-Alpes ARC1. Elias Hobeika is partly supported by SFB 1074 Project Z2. Stephen L Nutt was supported by the National Health and Medical Research Council of Australia (IRIISS grant # 361646, Program Grant # 1054925) and Victorian State Government Operational Infrastructure Support.