Journal article

Phenotype analysis of male transgenic mice overexpressing mutant IGFBP-2 lacking the Cardin-Weintraub sequence motif: Reduced expression of synaptic markers and myelin basic protein in the brain and a lower degree of anxiety-like behaviour

N Schindler, J Mayer, S Saenger, U Gimsa, C Walz, J Brenmoehl, D Ohde, E Wirthgen, A Tuchscherer, VC Russo, M Frank, T Kirschstein, F Metzger, A Hoeflich



Brain growth and function are regulated by insulin-like growth factors I and II (IGF-I and IGF-II) but also by IGF-binding proteins (IGFBPs), including IGFBP-2. In addition to modulating IGF activities, IGFBP-2 interacts with a number of components of the extracellular matrix and cell membrane via a Cardin-Weintraub sequence or heparin binding domain (HBD1). The nature and the signalling elicited by these interactions are not fully understood. Here, we examined transgenic mice (H1d-hBP2) overexpressing a mutant human IGFBP-2 that lacks a specific heparin binding domain (HBD1) known as the Cardin-Weintraub sequence. H1d-hBP2 transgenic mice have the genetic background of FVB mice and are char..

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Funding Acknowledgements

The authors are indebted to Luong Chau, Sabine Hinrichs, Marion Spitschak, the Team of the LIN Dummerstorf and Martina Pohlmann for excellent animal care and technical support. This work was supported by a grant from the Deutsche Forschungsgemeinschaft (DFG HO 2003/6-1). We further acknowledge generation of the transgenic mouse models used in our study by Tamara Holy and Prof. Eckhard Wolf (Institute of Molecular Animal Breeding, Gene Center, LMU Munich, Germany). The publication of this article was funded by the Open Access fund of the Leibniz Institute for Farm Animal Biology (FBN).