Rbf Regulates Drosophila Spermatogenesis via Control of Somatic Stem and Progenitor Cell Fate in the Larval Testis
Nicole Dominado, John E La Marca, Nicole A Siddall, James Heaney, Mai Tran, Yu Cai, Fengwei Yu, Hongyan Wang, W Gregory Somers, Leonie M Quinn, Gary R Hime
STEM CELL REPORTS | CELL PRESS | Published : 2016
The Drosophila testis has been fundamental to understanding how stem cells interact with their endogenous microenvironment, or niche, to control organ growth in vivo. Here, we report the identification of two independent alleles for the highly conserved tumor suppressor gene, Retinoblastoma-family protein (Rbf), in a screen for testis phenotypes in X chromosome third-instar lethal alleles. Rbf mutant alleles exhibit overproliferation of spermatogonial cells, which is phenocopied by the molecularly characterized Rbf11 null allele. We demonstrate that Rbf promotes cell-cycle exit and differentiation of the somatic and germline stem cells of the testes. Intriguingly, depletion of Rbf specifical..View full abstract
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Awarded by NHMRC
The authors wish to thank Shigeo Hayashi, Bruce Edgar, Bob Duronio, and Ting Xie for Drosophila strains. This work was funded by NHMRC Project grants to G.H. (APP1048110) and L.M.Q. (APP628414) and an NHMRC postdoctoral fellowship to W.G.S.