Neurons and oligodendrocytes in the mouse brain differ in their ability to replicate Semliki Forest virus
Rennos Fragkoudis, Nele Tamberg, Ricky Siu, Kaja Kiiver, Alain Kohl, Andres Merits, John K Fazakerley
Journal of NeuroVirology | SPRINGER | Published : 2009
Semliki Forest virus (SFV) provides an experimental model of acute virus encephalitis and virus-induced demyelinating disease. Two marker viruses expressing fluorescent proteins as part of the replicase or the structural open reading frame were used to evaluate virus replication in cells of the adult mouse brain. Both marker viruses established a high-titer infection in the adult mouse brain. As determined by location, morphology, and immunostaining with neural cell type-specific phenotypic markers, both viruses infected neurons and oligodendrocytes but not astrocytes. Determination of eGFP expression from either the replicase or the structural open-reading frame coupled with immunostaining ..View full abstract
This work was funded by the EU SFVECTORS (www.sfvectors.ed.ac.uk) programme.