Journal article

MEASUREMENT OF THYROID-STIMULATING IMMUNOGLOBULINS BY INCORPORATION OF TRITIATED-ADENINE INTO INTACT FRTL-5 CELLS - A VIABLE ALTERNATIVE TO RADIOIMMUNOASSAY FOR THE MEASUREMENT OF CAMP

PJ LEEDMAN, AG FRAUMAN, PG COLMAN, VP MICHELANGELI

Clinical Endocrinology | WILEY-BLACKWELL | Published : 1992

Abstract

OBJECTIVE: To evaluate the utility of 3H-adenine incorporation into intact rat thyroid epithelial cells (FRTL-5) as an alternative to radioimmunoassay for the measurement of cAMP following stimulation of these cells by serum thyroid-stimulating immunoglobulins from patients with Graves' disease. DESIGN: We determined the cAMP produced by FRTL-5 cells following incubation with serum from patients with a spectrum of autoimmune thyroid and other diseases using the 3H-adenine assay. PATIENTS: We studied 27 patients with untreated Graves' disease, 10 with Graves' disease complicated by ophthalmopathy (all on antithyroid medication), 11 with Hashimoto's thyroiditis, five with multinodular goitre, ..

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