Journal article

STRUCTURE OF INSULIN INSULIN-LIKE GROWTH FACTOR-I RECEPTORS ON THE INSULINOMA CELL, RIN-M5F

PG COLMAN, LC HARRISON

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Published : 1984

Abstract

The subunit structure of receptors for insulin and insulin-like growth factor-1 has been demonstrated on a rat insulinoma cell, RIN-m5F, grown in continuous culture. The specific binding of [125-I]-insulin-like growth factor-1 (2.9 +/- 0.42%; mean +/- SEM) to the RIN-m5F cell was significantly greater than of [125I]-insulin (0.7 +/- 0.2%). Immunoprecipitation of receptors from [125I]-surface labeled cells and analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography revealed reduced subunits of Mr 130,000 and 90,000, identical to those for both insulin and insulin-like growth factor-1 receptors on classic target tissues. An apparent abundance of insulin-like g..

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